O40 Determination of the global gene expression profile of oral cancers using paraffin-embedded tissues
نویسندگان
چکیده
منابع مشابه
Detection of Survivin Gene Expression in Formalin-Fixed Paraffin-Embedded Tissues of Human Osteosarcoma: Its Potential Usefulness in Diagnosis and Prognosis of Bone Tumors
Osteosarcoma is a relatively uncommon malignancy however, it is the most common form of primary malignant bone tumors in human. Diagnosis and prognosis of patients with osteosarcoma is limited to clinico-radiopathological parameters, whereas molecular markers of tumor aggression have been poorly identified. Survivin, an inhibitor of apoptosis (IAP), is unique for its expression in human tumors ...
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Quantitative gene expression profiling in formalin-fixed, paraffin-embedded tissues using universal bead arrays.
We recently developed a sensitive and flexible gene expression profiling system that is not dependent on an intact poly-A tail and showed that it could be used to analyze degraded RNA samples. We hypothesized that the DASL (cDNA-mediated annealing, selection, extension and ligation) assay might be suitable for the analysis of formalin-fixed, paraffin-embedded tissues, an important source of arc...
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15 صفحه اولDirect quantification of gene expression in homogenates of formalin-fixed, paraffin-embedded tissues.
Formalin-fixed, paraffin-embedded (FFPE) tissues represent an important source of archival materials for gene expression profiling. We report here the development of a modified branch DNA assay that allows direct quantification of messenger RNA (mRNA) transcripts in homogenates from FFPE tissue sections without the need for RNA isolation and reverse transcription into cDNA. Formalin fixation es...
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ژورنال
عنوان ژورنال: Oral Oncology Supplement
سال: 2007
ISSN: 1744-7895
DOI: 10.1016/s1744-7895(07)70066-4